Description
It is a methodology utilized to segregate chemical compounds according to their varied affinities towards a fixed phase and a mobile phase. The fixed phase typically consists of a solid substrate enclosed within a column, while the mobile phase typically comprises a liquid or a blend of liquids.
Usage of HPLC
HPLC used to separate components of an organic mixture of compounds when these compounds are non-volatile, thermally unstable and have relatively high molecular weights.
